Strontium ranelate improves alveolar bone healing in estrogen‐deficient rats

Abstract

Background

This study evaluated the impact of strontium ranelate on tooth‐extraction wound healing in estrogen‐deficient and estrogen‐sufficient rats.

Methods

Ninety‐six Wistar rats (90 days of age) were allocated into one of the following groups: sham‐surgery+water (estrogen‐sufficient); ovariectomy+water (estrogen‐deficient), sham‐surgery+strontium ranelate (625 mg/kg/d) (strontium/estrogen‐sufficient); ovariectomy+strontium ranelate (625 mg/kg/d) (strontium/estrogen‐deficient). Water or strontium ranelate were administrated from the 14th day post‐ovariectomy/sham surgery until euthanasia. Maxillary first molars were extracted at 21 days after sham/ovariectomy surgery. Rats were euthanized at 10, 20, and 30 days post‐extractions. The following parameters were analyzed inside tooth‐extraction wound: proportion of newly formed bone (bone healing/BH), number of cells stained for tartrate‐resistant acid phosphatase (TRAP) and immunohistochemical staining for five bone metabolism‐related markers (osteocalcin [OCN], osteopontin [OPN], bone sialoprotein [BSP], osteoprotegerin [OPG] and receptor activator of NF‐КB ligand [RANKL]).

Results

The estrogen‐deficient group presented lower BH than all other groups at 20 and 30 days post‐extraction (P < 0.05). The number of TRAP‐stained cells was higher in the estrogen‐deficient group than in estrogen‐sufficient group at 30 days post‐extraction (P < 0.05). The strontium /estrogen‐sufficient group exhibited stronger staining for OCN, when compared to the estrogen‐sufficient and estrogen‐deficient groups (P < 0.05). Both strontium ranelate‐treated groups presented higher staining of OPN and BSP than both untreated groups (P < 0.05). The strontium/estrogen‐sufficient group demonstrated stronger staining for OPG than the estrogen‐deficient group (P < 0.05). The estrogen‐sufficient group and both groups treated with strontium ranelate showed lower expression of RANKL than the estrogen‐deficient group (P < 0.05).

Conclusions

Strontium ranelate benefited BH and the expression of bone markers in tooth‐extraction wound in estrogen‐deficient rats whereas its benefits in estrogen‐sufficient rats were modest.

from
https://aap.onlinelibrary.wiley.com/doi/abs/10.1002/JPER.19-0561?af=R